Work

Cryo-Electron Tomographic Structure of an Immunodeficiency Virus Envelope Complex In Situ

Year: 2006

Type: article

Abstract: The envelope glycoprotein (Env) complexes of the human and simian immunodeficiency viruses (HIV and SIV, respectively) mediate viral entry and are a target for neutralizing antibodies. The receptor binding surfaces of Env are in large part sterically occluded or conformationally masked prior to receptor binding. Knowledge of the unliganded, trimeric Env structure is key for an understanding of viral entry and immune escape, and for the design of vaccines to elicit neutralizing antibodies. We have used cryo-electron tomography and averaging to obtain the structure of the SIV Env complex prior to fusion. Our result reveals novel details of Env organisation, including tight interaction between monomers in the gp41 trimer, associated with a three-lobed, membrane-distal gp120 trimer. A cavity exists at the gp41-gp120 trimer interface. Our model for the spike structure agrees with previously predicted interactions between gp41 monomers, and furthers our understanding of gp120 interactions within an intact spike.

Source: PLoS Pathogens

DOI: 10.1371/journal.ppat.0020083

Authors: Giulia Zanetti, John A. G Briggs, Kay Grünewald, Quentin J Sattentau, Stephen D Fuller

Institutions: Centre for Human Genetics, University of Oxford, Ludwig-Maximilians-Universität München, Max Planck Society, Max Planck Institute of Biochemistry

Language: English

Topic: HIV Research and Treatment

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,043