Work

Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses

Year: 2011

Type: article

Abstract: We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.

Source: Optics Express

DOI: 10.1364/oe.19.003130

Authors: Johanna Bückers, Dominik Wildanger, Giuseppe Vicidomini, Lars Kastrup, Stefan W. Hell

Institutions: Max Planck Institute for Biophysical Chemistry, Max Planck Society

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,270

OpenAlex ID: W1991219220

FWCI: 14.44

Cites: 30

Cited by: 233

Related to: 10