Work

Stimulation and partial stabilization of human histidyl‐tRNA synthetase by hemoglobin

Year: 1988

Type: article

Abstract: Histidyl-tRNA synthetase, an enzyme against which antibodies are directed in some patients with polymyositis, has been purified 5000-fold from HeLa cells, but was extremely labile to dilution or on storage at -80 degrees C. In order to facilitate study of the biochemical and immunological properties of the enzyme, a stabilizer was sought. Hemoglobin at 2 mg/ml was found to stimulate the enzyme and also partially preserved the activity of the enzyme stored at a low concentration (less than 10 micrograms/ml). Hematin, but not the globin protein, could substitute for hemoglobin in stimulating the enzyme.

Source: FEBS Letters

DOI: 10.1016/0014-5793(88)80827-5

Authors: Tapas Biswas, Frederick W. Miller, Paul H. Plotz

Institutions: National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Institutes of Health

OpenAlex ID: W1991495211

FWCI: 0.395

Cites: 15

Cited by: 4

Related to: 10