Work

Detection of apolipoprotein E phenotype in unconcentrated cerebrospinal fluid

Year: 2003

Type: article

Abstract: We developed a simple method to detect apolipoprotein E (Apo E) polymorphism distribution in approximately 20 microL of unconcentrated cerebrospinal fluid (CSF). A combination of isoelectric focusing in 3 M urea gel and immunoblotting was employed. Apo E phenotypes were identified in CSF samples from 45 patients with probable Alzheimer disease (AD), 15 with multiple sclerosis (MS), and 25 with other neurological diseases (OND). When the data were compared with a set of matched plasma samples, the results were identical. The method is useful for Apo E phenotyping from fresh or frozen unconcentrated CSF, when blood or plasma is not available.

Source: Journal of Clinical Laboratory Analysis

DOI: 10.1002/jcla.10063

Authors: P.D. Mehta, B.A. Patrick, T. Pirttila, P.K. Coyle, P.S. Aisen

Institutions: Kuopio University Hospital, State University of New York, Stony Brook University, Georgetown University Medical Center

Language: English

Topic: Alzheimer's disease research and treatments

Subfield: Physiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W1992089057

FWCI: 0.544

Cites: 26

Cited by: 7

Related to: 10