Work

Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytes

Year: 1989

Type: article

Abstract: Micro-injection of, or incubation with okadaic acid (OA), a specific phosphatase inhibitor, can induce formation of maturation-promoting factor (MPF) and germinal vesicle breakdown (GVBD) in Xenopus laevis oocytes. Comparison of the dose-response curves of OA on maturation, isolated enzymes and phosphatase activities in crude oocyte preparations suggests that inhibition of both polycation-stimulated (PCS) and ATP,Mg-dependent (AMD) phosphatases is sufficient but requires that a critical phosphorylation level is attained of one or several of their substrates, resulting in the formation of active MPF and meiotic maturation.

Source: FEBS Letters

DOI: 10.1016/0014-5793(89)80198-x

Authors: Jozef Goris, Jacques Hermann, Peter Hendrix, René Ozon, Wilfried Merlevede

Institutions: KU Leuven, Université Pierre-et-Marie-Curie

Language: English

Topic: Reproductive Biology and Fertility

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

OpenAlex ID: W1992529920

FWCI: 17.09

Cites: 29

Cited by: 180

Related to: 10