Work

Electronic Barcoding of a Viral Gene at the Single-Molecule Level

Year: 2012

Type: article

Abstract: A new single-molecule approach for rapid and purely electronic discrimination among similar genes is presented. Combining solid-state nanopores and γ-modified synthetic peptide nucleic acid probes, we accurately barcode genes by counting the number of probes attached to each gene and measuring their relative spacing. We illustrate our method by sensing individual genes from two highly similar human immunodeficiency virus subtypes, demonstrating feasibility of a novel, single-molecule diagnostic platform for rapid pathogen classification.

Source: Nano Letters

DOI: 10.1021/nl300372a

Authors: Alon Singer, Srinivas Rapireddy, Danith H. Ly, Amit Meller

Institutions: Boston University, Carnegie Mellon University

Language: English

Topic: Nanopore and Nanochannel Transport Studies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funder: National Human Genome Research Institute

Award: G1978350919