Somatic Embryogenesis and Plant Regeneration from Hypocotyl and Leaf Explants of Brassica Oleracea var. Botrytis (Cauliflower)
Year: 2011
Type: article
Abstract: We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var.botrytis (cauliflower), a very important vegetable crop worldwide.Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var.botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin.Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l).Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D.We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary).More embryos per explant were produced through the indirect pathway (23-25) than through the direct pathway (14-19).The number of embryos produced was high.There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species.Plant regeneration was achieved on half-strength MS medium without any hormones.
Authors: Poon Siong, Rosna Taha, Fatimah Rahiman
Institutions: University of Malaya, Jagiellonian University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W1993160620