Work

Rapid modification of bacterial artificial chromosomes by ET- recombination

Year: 1999

Type: article

Abstract: We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

Source: Nucleic Acids Research

DOI: 10.1093/nar/27.6.1555

Authors: J. Muyrers, Y Zhang, G Testa, A F Stewart

Institutions: European Molecular Biology Laboratory, European Molecular Biology Laboratory

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1993330603

FWCI: 5.554

Cites:

Cited by: 535

Related to: 10