Work

Characterization of high‐affinity protein D binding sites on the surface of rat epididymal spermatozoa

Year: 1996

Type: article

Abstract: 125I-labeled protein D was shown to bind to whole rat epididymal sperm in a time-dependent, saturable, and reversible manner. Scatchard analysis indicates that there are 3.4 x 10(5) binding sites/sperm cell with a binding efficiency (Kd) of 2.3 x 10(-10) M. Binding of protein D to sperm reaches equilibrium by 95 min. and is reversible by 30 min. Specific binding of protein D to sperm pre-treated with a dilute trypsin solution is reduced significantly when compared with controls. Our findings establish that protein D-specific binding proteins displaying characteristics of a true receptor are present on the surface of rat epididymal sperm.

Source: IUBMB Life

DOI: 10.1080/15216549600201633

Authors: Joseph C. Hall, Christopher E. Tubbs, Yuehua Li, Salman Ashraf

Institution: North Carolina State University

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funders: National Science Foundation, Claude Pepper Older Americans Independence Center, Wake Forest School of Medicine

Award: Presidential Young Investigator Award