Work

Colorimetric Cu2+ Detection Using DNA‐Modified Gold‐Nanoparticle Aggregates as Probes and Click Chemistry

Year: 2010

Type: article

Abstract: The colorimetric detection of Cu2+ is demonstrated using DNA-modified gold nanoparticle (Au NP) aggregates as probes and a copper-catalyzed click reaction to initiate the cross-coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.

Source: Small

DOI: 10.1002/smll.200901691

Authors: Xiaoyang Xu, Weston L. Daniel, Wei Wei, Chad A. Mirkin

Institutions: Northwestern University, International Institute for Nanotechnology

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G9000165630