Work

High-speed multispectral confocal biomedical imaging

Year: 2014

Type: article

Abstract: A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.jbo.19.3.036016

Authors: Gary E. Carver, Sarah A. Locknar, William A. Morrison, V. Krishnan Ramanujan, Daniel L. Farkas

Institutions: Omega Optics (United States), Cedars-Sinai Medical Center, University of Southern California

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funders: Cedars-Sinai Medical Center, National Institutes of Health, National Cancer Institute

Award: Rapid In-vivo Cancer Detection via Multispectral Confocal Cellular Imaging