Work

Sub‐classification of response regulators using the surface characteristics of their receiver domains

Year: 2003

Type: article

Abstract: The omnipresent bacterial switch known as a two-component system is comprised of a response regulator and a sensor kinase with which it interacts. Sensor kinases have been classified and further sub-classified into groups based on their sequence similarity, loop lengths and domain organization. Response regulators have been classified predominantly by the identity and function of their output domains. Here, comparative based homology modeling of the receiver domains of the OmpR sub-family of response regulators in Bacillus subtilis and Escherichia coli suggests further sub-classification is possible. A color-coded scale is used to show trends in surface hydrophobicity. For the OmpR receiver domains modeled these trends allow further sub-classification. The specific surface regions used for this sub-classification procedure correlate with clusters of residues that are important for interaction with cognate four helix bundle HisKA/Hpt domains.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(03)01167-0

Authors: Douglas J Kojetin, Richele J Thompson, John Cavanagh

Institution: North Carolina State University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G5143764922, NMR Studies of Bacterial Regulatory Proteins