Work

Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.

Year: 1984

Type: article

Abstract: Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was increased by the products of both the 13S and the 12S RNAs. By measuring the level of specific transcription in nuclei isolated from transfected cells we directly demonstrate that the increased amount of EIIa RNA is due to stimulation of the rate of transcription.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.81.14.4381

Authors: T Leff, R Elkaim, C R Goding, P Jalinot, P Sassone-Corsi, M Perricaudet, C Kédinger, P Chambon

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1995012997

FWCI: 14.56

Cites: 36

Cited by: 124

Related to: 10