Origin-Dependent Neural Cell Identities in Differentiated Human iPSCs In Vitro and after Transplantation into the Mouse Brain
Year: 2014
Type: article
Abstract: The differentiation capability of induced pluripotent stem cells (iPSCs) toward certain cell types for disease modeling and drug screening assays might be influenced by their somatic cell of origin. Here, we have compared the neural induction of human iPSCs generated from fetal neural stem cells (fNSCs), dermal fibroblasts, or cord blood CD34(+) hematopoietic progenitor cells. Neural progenitor cells (NPCs) and neurons could be generated at similar efficiencies from all iPSCs. Transcriptomics analysis of the whole genome and of neural genes revealed a separation of neuroectoderm-derived iPSC-NPCs from mesoderm-derived iPSC-NPCs. Furthermore, we found genes that were similarly expressed in fNSCs and neuroectoderm, but not in mesoderm-derived iPSC-NPCs. Notably, these neural signatures were retained after transplantation into the cortex of mice and paralleled with increased survival of neuroectoderm-derived cells in vivo. These results indicate distinct origin-dependent neural cell identities in differentiated human iPSCs both in vitro and in vivo.
Source: Cell Reports
Authors: Gunnar Hargus, Marc Ehrlich, Marcos J. Araúzo-Bravo, Kathrin Hemmer, Anna-Lena Hallmann, Peter Reinhardt, Kee-Pyo Kim, Kenjiro Adachi, Simeon Santourlidis, Foued Ghanjati, Mareike Fauser, Christiana Ossig, Alexander Storch, Jeong Beom Kim, Jens C. Schwamborn, Jared Sterneckert, Hans R. Schöler, Tanja Kuhlmann, Holm Zaehres
Institutions: University Hospital Münster, Max Planck Institute for Molecular Biomedicine, Ikerbasque, Biogipuzkoa Health Research Institute, University of Luxembourg, University of Münster, Luxembourg Centre for Systems Biomedicine, Heinrich Heine University Düsseldorf, Center for Regenerative Therapies Dresden, Technische Universität Dresden, Ulsan National Institute of Science and Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,620
Awards: G1347414165, G2304404308, G2319492348, G2529282916, G2540087163, G2645499977, Verbundprojekt: neurohiPS - Derivate humaner neuraler Stammzellen: Von der Multipotenz zur Pluripotenz, Teilprojekt 4: Induktion pluripotenter Stammzellen aus humanen palatalen Stammzellen der Neuralleiste, G5535037449, G6050889818, G6753010118, G795160076
OpenAlex ID: W1995240811