Work

Arachidonic andcis‐unsaturated fatty acids induce selective platelet substrate phosphorylation through activation of cytosolic protein kinase C

Year: 1991

Type: article

Abstract: The ability of arachidonic acid and other fatty acids to induce phosphorylation of endogenous substrates and the role of protein kinase C in mediating these effects were examined. In a cell-free cytosolic system derived from human platelets, arachidonic, oleic, and other cis-unsaturated fatty acids induced a dose-dependent phosphorylation of several endogenous substrates. These substrates form a subset of phorbol ester-induced phosphorylations. Multiple lines of evidence suggested the direct involvement of protein kinase C in mediating fatty acid-induced phosphorylations. These observations suggest that arachidonic acid and other unsaturated fatty acids are capable of activating protein kinase C in a physiologic environment resulting in the phosphorylation of multiple endogenous substrates.

Source: FEBS Letters

DOI: 10.1016/0014-5793(91)80843-r

Authors: W Khan, S el Touny, Y A Hannun

Institutions: Duke Medical Center, Duke University

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Heart, Lung, and Blood Institute, National Cancer Institute

Awards: G1974877716, G699147582