Work

The sequence of human serum albumin cDNA and its expression in E. coli

Year: 1981

Type: article

Abstract: A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control of the E. coli trp promoter-operator. The DNA sequence and predicted protein sequence of HSA were determined from the cDNA plasmid and are compared to existing data obtained from direct protein sequencing. The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid "prepro" peptide.

Source: Nucleic Acids Research

DOI: 10.1093/nar/9.22.6103

Authors: Richard M. Lawn, John Adelman, Susan C. Bock, Arthur E. Franke, Catherine M. Houck, Richard Najarian, Peter H. Seeburg, Karen L. Wion

Institution: Genentech

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1996099693

FWCI: 5.628

Cites: 35

Cited by: 200

Related to: 9