Targeted gene disruption to cure HIV
Year: 2013
Type: article
Abstract: PURPOSE OF REVIEW: Recent clinical research suggests that an HIV-infected patient with lymphoma who was transplanted with bone marrow homozygous for a disrupted mutant CCR5 allele has no remaining HIV replication and is effectively cured of HIV. Here, we discuss the approaches of disrupting host and viral genes involved in HIV replication and pathogenesis with the aim of curing patients with HIV. RECENT FINDINGS: Data from the 'Berlin patient' suggest that targeted gene disruption can lead to an HIV cure. This review discusses the recent advances in the field of gene disruption toward the development of an anti-HIV therapy. We will introduce the strategies to disrupt host and viral genes using precise disruptions, imprecise disruptions, or site-specific recombination. Furthermore, the production of engineered rare-cutting endonucleases (zinc finger nucleases, TAL effector nucleases, and homing endonucleases) and recombinases that can recognize specific DNA target sequences and facilitate gene disruption will be discussed. SUMMARY: The discovery of a gene disruption approach that would cure or efficiently confine HIV infection could have broad implications for the treatment of millions of people infected with HIV. An efficient 'one-shot' curative therapy not only would give infected patients hope of a drug-free or treatment-free future, but also could reduce the huge financial burden faced by many countries because of widespread administration of highly active antiretroviral therapy.
Source: Current Opinion in HIV and AIDS
Authors: Daniel Stone, Hans-Peter Kiem, Keith R. Jerome
Institutions: University of Washington, Fred Hutch Cancer Center
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Awards: Targeted Modification of Host and Proviral DNA to Treat Latent HIV Infection, LAGLIDADG Delivery and Gene Repair in Hematopoietic Stem Cells (7 of 11), G5007394730, Development of Novel AIDS Gene Therapy Strategies in the Macaque SHIV Model, University of Washington/Fred Hutch Center for AIDS Research
OpenAlex ID: W1996175658