Structure of the first representative of Pfam family PF04016 (DUF364) reveals enolase and Rossmann-like folds that combine to form a unique active site with a possible role in heavy-metal chelation
Year: 2010
Type: article
Abstract: The crystal structure of Dhaf4260 from Desulfitobacterium hafniense DCB-2 was determined by single-wavelength anomalous diffraction (SAD) to a resolution of 2.01 Å using the semi-automated high-throughput pipeline of the Joint Center for Structural Genomics (JCSG) as part of the NIGMS Protein Structure Initiative (PSI). This protein structure is the first representative of the PF04016 (DUF364) Pfam family and reveals a novel combination of two well known domains (an enolase N-terminal-like fold followed by a Rossmann-like domain). Structural and bioinformatic analyses reveal partial similarities to Rossmann-like methyltransferases, with residues from the enolase-like fold combining to form a unique active site that is likely to be involved in the condensation or hydrolysis of molecules implicated in the synthesis of flavins, pterins or other siderophores. The genome context of Dhaf4260 and homologs additionally supports a role in heavy-metal chelation.
Authors: Mitchell D. Miller, L. Aravind, Constantina Bakolitsa, Christopher L. Rife, Dennis Carlton, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Hsiu-Ju Chiu, Thomas Clayton, Marc C. Deller, Lian Duan, Julie Feuerhelm, Joanna C. Grant, Gye Won Han, Lukasz Jaroszewski, Kevin K. Jin, Heath E. Klock, Mark W. Knuth, Piotr Kozbial, S. Sri Krishna, Abhinav Kumar, David Marciano, Daniel McMullan, Andrew T. Morse, Edward Nigoghossian, Linda Okach, Ron Reyes, Henry van den Bedem, Dana Weekes, Qingping Xu, Keith O. Hodgson, John Wooley, Marc-André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson
Institutions: SLAC National Accelerator Laboratory, Joint Center for Structural Genomics, Stanford Synchrotron Radiation Lightsource, National Institutes of Health, Sanford Burnham Prebys Medical Discovery Institute, Scripps Research Institute, Genomics Institute of the Novartis Research Foundation, University of California San Diego
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W1996198439