Multicolor Far-Field Fluorescence Nanoscopy through Isolated Detection of Distinct Molecular Species
Year: 2008
Type: article
Abstract: By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
Source: Nano Letters
DOI: 10.1021/nl801471d
Authors: Mariano Bossi, Jonas Fölling, Vladimir N. Belov, Vadim P. Boyarskiy, Rebecca Medda, Alexander Egner, Christian Eggeling, Andreas Schönle, Stefan W. Hell
Institution: Max Planck Institute for Biophysical Chemistry
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W1996375666