Work

Membrane protein assembly into Nanodiscs

Year: 2009

Type: review

Abstract: Nanodiscs are soluble nanoscale phospholipid bilayers which can self-assemble integral membrane proteins for biophysical, enzymatic or structural investigations. This means for rendering membrane proteins soluble at the single molecule level offers advantages over liposomes or detergent micelles in terms of size, stability, ability to add genetically modifiable features to the Nanodisc structure and ready access to both sides of the phospholipid bilayer domain. Thus the Nanodisc system provides a novel platform for understanding membrane protein function. We provide an overview of the Nanodisc approach and document through several examples many of the applications to the study of the structure and function of integral membrane proteins.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2009.10.024

Authors: Timothy H. Bayburt, Stephen G. Sligar

Institutions: University of Illinois Urbana-Champaign, University of Illinois System

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G4440624827, Metalloenzyme Mechanisms, G535672703, HEME Protein Reductases, G8455228585