Work

A CRISPR view of development

Year: 2014

Type: preprint

Abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.

Source: Genes & Development

DOI: 10.1101/gad.248252.114

Authors: Melissa M. Harrison, Brian V. Jenkins, Kate M. O’Connor-Giles, Jill Wildonger

Institution: University of Wisconsin–Madison

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: March of Dimes Foundation, University of Wisconsin-Madison, National Institutes of Health, National Institute of Neurological Disorders and Stroke

Awards: G3073152869, G3456500988, CRISPR/Cas9 tools for identifying and manipulating diverse neuronal populations