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Intracellular ADP activates K+ channels that are inhibited by ATP in an insulin‐secreting cell line

Year: 1986

Type: article

Abstract: The effect of ADP on ATP-sensitive K+ channels in the insulin-secreting RINm5F cell line has been investigated with the help of single-channel current recording from saponin-permeabilized cells. ADP (100-500 microM) markedly activates K+ channels when added to the bath solution in contact with the membrane inside. ADP-beta-S cannot mimick this effect. During sustained ATP (500 microM)-evoked inhibition of K+ channel opening, 500 microM ADP markedly and reversibly activates the channels. Conversely ATP markedly reduces the opening probability of ADP-activated channels. It is suggested that the physiological control of K+ channel opening in the insulin-secreting cells is mediated by changes in ATP/ADP ratio rather than being solely determined by the ATP concentration.

Source: FEBS Letters

DOI: 10.1016/0014-5793(86)81532-0

Authors: Mark J. Dunne, Ole H. Petersen

Institution: University of Liverpool

Language: English

Topic: Ion channel regulation and function

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Université de Genève, Medical Research Council

OpenAlex ID: W1997849672

FWCI: 4.04

Cites: 19

Cited by: 225

Related to: 10