Work

Cholesterol-Phospholipid Interactions: New Insights from Surface X-Ray Scattering Data

Year: 2010

Type: article

Abstract: We report a structural study of cholesterol-DPPC (1,2-dipalmitoyl-sn-glycero-3-phophocholine) monolayers using x-ray reflectivity and grazing incidence x-ray diffraction. Reflectivity reveals that the vertical position of cholesterol relative to phospholipids strongly depends on its mole fraction (chi(CHOL)). Moreover, we find that at a broad range of chi(CHOL) cholesterol and DPPC form alloylike mixed domains of short-range order and the same stoichiometry as that of the film. Based on the data presented, we propose a new model of cholesterol-phospholipid organization in mixed monolayers.

Source: Physical Review Letters

DOI: 10.1103/physrevlett.104.108101

Authors: Andrey Ivankin, Ivan Kuzmenko, David Gidalevitz

Institutions: Illinois Institute of Technology, Argonne National Laboratory, Advanced Photon Source

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Award: Antimicrobial Peptides and their Synthetic Mimics - Investigating the Mechanism o