Work

Nuclear Translocation of Extradenticle Requires , which Encodes an Extradenticle-Related Homeodomain Protein

Year: 1997

Type: article

Abstract: We show that homothorax (hth) is required for the Hox genes to pattern the body of the fruit fly, Drosophila melanogaster. hth is necessary for the nuclear localization of an essential HOX cofactor, Extradenticle (EXD), and encodes a homeodomain protein that shares extensive identity with the product of Meis1, a murine proto-oncogene. MEIS1 is able to rescue hth mutant phenotypes and can induce the cytoplasmic-to-nuclear translocation of EXD in cell culture and Drosophila embryos. Thus, Meis1 is a murine homolog of hth. MEIS1/HTH also specifically binds to EXD with high affinity in vitro. These data suggest a novel and evolutionarily conserved mechanism for regulating HOX activity in which a direct protein-protein interaction between EXD and HTH results in EXD's nuclear translocation.

Source: Cell

DOI: 10.1016/s0092-8674(00)80400-6

Authors: Gabrielle E Rieckhof, Fernando Casares, Hyung Don Ryoo, Muna Abu-Shaar, Richard S Mann

Institution: Columbia University

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $11,400

Funder: National Institute of General Medical Sciences