Work

[18F]-Fhbg-PET/CT Imaging of CD34-TK75+ T Cells in Allogeneic HSCT Recipients after Donor Lymphocyte Infusion (IND#11917; Clinicaltrials.Gov Identifier: NCT00871702)

Year: 2014

Type: article

Abstract: Allogeneic transplantation is associated with both GvL and GvHD. Elimination of donor T cells results in less GvHD, but also less GvL and engraftment. To eradicate infused T cells if GvHD develops, our suicide gene based therapy uses a CD34-Herpes Simplex Virus-1-thymidine kinase (CD34-TK75) chimera (Rettig et al, Mol Ther 2003 and J Immunol 2006). We enrich and purify retrovirally transduced cells via cell surface expression of the extracellular domain of CD34. Modified TK75 both mediates cell suicide upon treatment with ganciclovir and enables tracking of modified cells that entrap the substrate, [18CT/CT.

Source: Transplantation and Cellular Therapy

DOI: 10.1016/j.bbmt.2013.12.197