Work

Photosynthetic pigments in 37 species (65 strains) of Haptophyta: implications for oceanography and chemotaxonomy

Year: 2004

Type: article

Abstract: The pigment compositions of 37 species (65 strains) of cultured haptophytes were analysed using improved HPLC methods.We distinguished 8 pigment types based on the distribution of 9 chlorophyll c (chl c) pigments and 5 fucoxanthin derivatives.All types contained chl c 2 and Mg-2, 4-divinyl phaeoporphyrin a 5 monomethyl ester (MgDVP), fucoxanthin, diadinoxanthin and β,βcarotene.Pigment types were based on the following additional pigments: Type 1: chl c 1 ; Type 2: chl c 1 and chl c 2 -Pavlova gyrans-type; Type 3: chl c 1 and chl c 2 -monogalactosyl diacylglyceride ester (chl c 2 -MGDG [18:4/14:0]); Type 4: chl c 1 , chl c 3 and non-polar chl c 1 -like; Type 5: chl c 1 , chl c 3 , chl c 2 -MGDG [18:4/14:0] and 4-keto-fucoxanthin; Type 6: chl c 3 , monovinyl chl c 3 (MV-chl c 3 ), chl c 2 -MGDG [18:4/14:0], 19'-hexanoyloxyfucoxanthin and its 4-keto derivative, and traces of 19'-butanoyloxyfucoxanthin; Type 7: similar to Type 6, minus MV-chl c 3 but with chl c 2 -MGDG [14:0/14:0] added; Type 8: similar to Type 6, minus MV-chl c 3 but with significant 19'-butanoyloxyfucoxanthin.Taxonomic associations ranged from single genera to multiple families -Type 1: Pavlovaceae, Isochrysidaceae and Pleurochrysidaceae; Type 2: Pavlovaceae; Type 3: Isochrysidaceae; Type 4: Prymnesium spp.; Type 5: Ochrosphaera spp.; Type 6: Nöelaerhabdaceae, notably Emiliania spp.; Type 7: Chrysochromulina spp.; Type 8: Phaeocystaceae, Prymnesiaceae and Isochrysidaceae.These pigment types showed a strong correlation with available phylogenetic trees, supporting a genetic basis for the pigment associations.The additional marker pigments offer oceanographers greater power for detecting haptophytes in mixed populations, while also distinguishing a greater proportion of them from diatoms.

Source: Marine Ecology Progress Series

DOI: 10.3354/meps270083

Authors: M Zapata, SW Jeffrey, SW Wright, F Rodríguez, JL Garrido, L Clementson