Systematic Domain Deletion Analysis of the Major Sporulation Kinase in Bacillus subtilis
Year: 2010
Type: article
Abstract: To characterize the role of the three PAS domains in KinA, the major sporulation kinase in Bacillus subtilis, we constructed a series of systematic PAS domain deletion mutants and analyzed their activities using an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible artificial sporulation induction system, which we have developed recently. The results showed that any one of the three PAS domains is sufficient to maintain the kinase activity and trigger sporulation, if not fully then at least partially, when the protein levels increase beyond a certain level.
Source: Journal of Bacteriology
DOI: 10.1128/jb.01481-09
Authors: Prahathees Eswaramoorthy, Masaya Fujita
Institution: University of Houston
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1999084729