Work

Synergistic Actions of a Monooxygenase and Cyclases in Aromatic Polyketide Biosynthesis

Year: 2008

Type: article

Abstract: Synergistic tailoring: Formation of the pentangular aglycon of pradimicin A requires the synergistic actions of the monooxygenase PdmH, which hydroxylates ring B, and the two cyclases PdmK and PdmL. Removal of any one of three enzymes resulted in the biosynthesis of shunt products that are not properly oxidized and cyclized. The concerted actions of the enzymes may serve as a mechanism to suppress spontaneous cyclization of the extended dodecaketide backbone.

Source: ChemBioChem

DOI: 10.1002/cbic.200800178

Authors: Jixun Zhan, Kenji Watanabe, Yi Tang

Institutions: University of California, Los Angeles, University of Southern California

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

Funders: National Science Foundation, Division of Chemical, Bioengineering, Environmental, and Transport Systems

Awards: G6671297155, MRI: Acquisition of High Field Nuclear Magnetic Resonance Instrumentation for Research on Nanochemistry, Purchase of Nuclear Magnetic Resonance Spectrometers, PECASE: Bioengineering of Secondary Metabolic Pathways