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Extraction and Reconstitution of Lyophilized Photosystem I Subchloroplast Fragments from Spinach Chloroplasts

Year: 1979

Type: article

Abstract: Lyophilized photosystem I subchloroplast fragments prepared from spinach chloroplasts were extracted and reconstituted. Hexane extraction eliminated 26 % of the photosystem I activity without removing chlorophylls, and the reconstitution with β-carotene, an unkown lipid or chlorophyll a restored almost all the activity. Extraction with hexane–acetone (2:1, v/v) eliminated 81% of the activity with removal of 78% of the chlorophylls. In this case, reconstitution with chlorophyll a showed complete restoration of the activity, but β-carotene, the unknown lipid, and plastoquinone A caused a rather inhibitory effect. A possible explanation is that photosystem I reaction centers are closely surrounded by chlorophyll a; and β-carotene, the unknown lipid, and plastoquinone A function only through chlorophyll a.

Source: Agricultural and Biological Chemistry

DOI: 10.1080/00021369.1979.10863602

Authors: Osamu Hirayama, Hideyuki Matsuda, Kanae Senzaki, Tsuneko Masuda

Institutions: Shimane University, University of Shimane

Language: English

Topic: Photosynthetic Processes and Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2000276592

FWCI:

Cites:

Cited by: 1

Related to: 10