Rab GTPase–Myo5B complexes control membrane recycling and epithelial polarization
Year: 2011
Type: article
Abstract: The Rab GTPases are the largest family of proteins regulating membrane traffic. Rab proteins form a nidus for the assembly of multiprotein complexes on distinct vesicle membranes to regulate particular membrane trafficking pathways. Recent investigations have demonstrated that Myosin Vb (Myo5B) is an effector for Rab8a, Rab10, and Rab11a, all of which are implicated in regulating different pathways for recycling of proteins to the plasma membrane. It remains unclear how specific interactions of Myo5B with individual Rab proteins can lead to specificity in the regulation of alternate trafficking pathways. We examined the relative contributions of Rab/Myo5B interactions with specific pathways using Myo5B mutants lacking binding to either Rab11a or Rab8a. Myo5B Q1300L and Y1307C mutations abolished Rab8a association, whereas Myo5B Y1714E and Q1748R mutations uncoupled association with Rab11a. Expression of Myo5B tails containing these mutants demonstrated that Rab11a, but not Rab8a, was required for recycling of transferrin in nonpolarized cells. In contrast, in polarized epithelial cyst cultures, Myo5B was required for apical membrane trafficking and de novo lumen formation, dependent on association with both Rab8a and Rab11a. These data demonstrate that different combinations of Rab GTPase association with Myo5B control distinct membrane trafficking pathways.
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Awards: Eunice Kennedy Shriver Intellectual and Developmental Disability Research Center, G1255095149, G1344704808, G150953014, G1939764788, G2019238319, G2304076072, G2940777275, G35732448, G3581829098, G3722609264, Molecular characteristics of the apical recycling system, G3989539122, Vanderbilt Mouse Metabolic Phenotyping Center, Recovery from acute kidney injury, G4513176822, G4524812950, G5072864676, Mucosal Immune Barrier in Infection and Immunity, G6401783521, G699381380, G725763919, Diabetes Research and Training Center, Molecular and Cellular Basis for Digestive Diseases, Vanderbilt Diabetes Research Center, Small GTP Binding Proteins in Gastrointestinal Mucosa, Core Grant in Vision Research
OpenAlex ID: W2000770367