Work

From Structure to Sequence and Back Again

Year: 1996

Type: article

Abstract: With a simple lattice model and sequence design algorithm, we can design sequences to fit arbitrary compact globular structures. We judged the success of the design algorithm by performing exhaustive conformational searches to determine if a designed sequence's lowest energy conformation matched the target for which it was designed. Designed sequences tend to be much better optimized for their targets than a natural sequence is optimized for its lowest energy model conformation. We examined the effect of varying the number of available amino acid types on the success of the design method. It was more difficult but not impossible to successfully design discriminating sequences using fewer amino acid types.

Source: Journal of Molecular Biology

DOI: 10.1006/jmbi.1996.0243

Authors: David A. Hinds, Michael Levitt

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,730

Funders: National Institutes of Health, National Institute of General Medical Sciences