Work

Novel Design for Centrifugal Counter-current Chromatography: Iii. Saw Tooth Column

Year: 2010

Type: article

Abstract: The toroidal coil using an equilateral triangular core and zigzag pattern column have improved both retention of the stationary phase and peak resolution of the conventional toroidal coil in centrifugal counter-current chromatography. To further improve the retention of stationary phase and peak resolution, a novel saw tooth column was designed and the performance of the system was evaluated at various flow rates. The results indicated that both retention of the stationary phase and peak resolution were improved as the flow rate was decreased and at a flow rate of 0.005 ml/min the resolution is remarkably increased. Modification of the tubing called flat-twisted tubing further improved the peak resolution without increasing the column pressure. With a decreased column length at a capacity of about 0.2 ml, resolution of the saw tooth column was 1.02.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826071003684547

Authors: Yi Yang, Haji Akber Aisa, Yoichiro Ito

Institutions: National Institutes of Health, Chinese Academy of Sciences, Xinjiang Technical Institute of Physics & Chemistry, National Heart, Lung, and Blood Institute, University of Chinese Academy of Sciences

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute

Award: Novel column design for centrifugal countercurrent chromatography