Work

Covalently Linked Tandem Lesions in DNA

Year: 2012

Type: article

Abstract: Reactive oxygen species (ROS) generate a type of DNA damage called tandem lesions, two adjacent nucleotides both modified. A subcategory of tandem lesions consists of adjacent nucleotides linked by a covalent bond. Covalently linked tandem lesions generate highly characteristic liquid chromotography-tandem mass spectrometry (LC-MS/MS) elution profiles. We have used this property to comprehensively survey X-irradiated DNA for covalently linked tandem lesions. A total of 15 tandem lesions were detected in DNA irradiated in deoxygenated aqueous solution, five tandem lesions were detected in DNA that was irradiated in oxygenated solution.

Source: Radiation Research

DOI: 10.1667/rr2915.1

Authors: Helen B. Patrzyc, Jean B. Dawidzik, Edwin E. Budzinski, Harold G. Freund, John H. Wilton, Harold C. Box

Institution: Roswell Park Comprehensive Cancer Center

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: G4914835, Cancer Prevention/Risk Assessments from DNA Modification Levels, Roswell Park Cancer Center Support Grant