Work

Detection of Carbapenemase-Producing Bacteria by Using an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method

Year: 2013

Type: article

Abstract: The emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. This report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid detection of carbapenemase activity in enterobacteria, Pseudomonas aeruginosa, and Acinetobacter baumannii. In a panel of 78 isolates, including 41 carbapenemase-producing strains, the ULPC-MS/MS assay showed 100% agreement with molecular characterization, whereas six carbapenemase-producing isolates were not detected by the modified Hodge test.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.01540-13

Authors: Anne Carricajo, Paul O. Verhoeven, Salim Guezzou, Nathalie Fonsale, Gerald Aubert

Institutions: Université de Lyon, Centre Hospitalier Universitaire de Saint-Étienne

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Microbiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2001849897