Work

In Situ Monitoring of Backbone Thioester Exchange by 19F NMR

Year: 2009

Type: article

Abstract: 19F NMR to monitor BTE: A new strategy for assessing polypeptide tertiary structural stability by using thiol–thioester exchange is described. The backbone thioester exchange (BTE) method has previously been used to evaluate fold stability with HPLC measurements. Here we show that BTE can be followed by 19F NMR, which offers technical advantages. This technique was used for rapid identification of the most stable antiparallel coiled coil in a small library of new sequences.

Source: ChemBioChem

DOI: 10.1002/cbic.200900380

Authors: William C. Pomerantz, Erik B. Hadley, Charles G. Fry, Samuel H. Gellman

Institution: University of Wisconsin–Madison

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G2111837551, G4806206367