Work

Neutrophil Chemotaxis within a Competing Gradient of Chemoattractants

Year: 2012

Type: article

Abstract: The dynamics of neutrophil chemotaxis under competing chemoattractant gradients was studied using a microfluidic platform. This microfluidic platform, which establishes a stable and dynamic gradient of chemoattractants across a cell culture chamber, enabled the investigation of human neutrophil migration patterns in the presences of four different chemoattractants (leukotriene B(4), chemokine C-X-C motif ligands 2 and 8, and fMLP) and competing gradients of all pairwise combinations. The migration patterns for individual cells were tracked and quantitatively analyzed, and the results suggest a hierarchy among these chemoattractants of fMLP > CXCL8 > CXCL2 > leukotriene B(4). In all conditions, over 60% of neutrophils exposed to a competing gradient move toward the stronger signal though the weaker chemoattractant still influences neutrophil motility. These results yield insight about how each chemoattractant contributes to overall neutrophil chemotaxis within complex physiological environments.

Source: Analytical Chemistry

DOI: 10.1021/ac3009548

Authors: Donghyuk Kim, Christy L. Haynes

Institution: University of Minnesota

Language: English

Topic: Chemokine receptors and signaling

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Institutes of Health

Award: IMMUNE SYSTEM-ON-A-CHIP FOR QUANTITATIVE ANALYSIS OF CELL INTERACTIONS DURING ALL