Work

Cloning and functional characterization of a cocaine‐sensitive dopamine transporter

Year: 1991

Type: article

Abstract: We report the cloning of a rat cDNA encoding a functional dopamine transporter. This cDNA, derived from an intron-containing gene, encodes a protein of 620 amino acids. Hydropathicity analysis of the protein sequence suggests the presence of 12 putative transmembrane domains. The protein displays considerable identity with transporters for noradrenaline and GABA (64 and 30%, respectively). Transient expression of the cDNA in COS7 cells directs the expression of dopamine uptake activity with appropriate pharmacology and in a sodium-dependent fashion. In situ hybridization reveals that the mRNA for this transporter is expressed in the substantia nigra and ventral tegmental area, regions that contain dopaminergic cell bodies.

Source: FEBS Letters

DOI: 10.1016/0014-5793(91)81406-x

Authors: Bruno Giros, Salah El Mestikawy, Lucie Bertrand, Marc G. Caron

Institutions: Howard Hughes Medical Institute, Duke University, Duke Medical Center

Language: English

Topic: Neurotransmitter Receptor Influence on Behavior

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, Centers for Disease Control and Prevention, National Institute of Mental Health, National Institute of Neurological Disorders and Stroke