Work

Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries.

Year: 1991

Type: article

Abstract: A number of experiments on the shuffling of heavy and light chains from antibodies of defined specificity for the transition-state analogue hapten nitrophenyl phosphonamidate are described. The experiments report on the promiscuity of heavy and light chains in binding antigen and the feasibility of antibody redesign by this shuffling process. The concepts of incestuous and extraclonal promiscuous association are described. Shuffling opens the possibility of generating panels of antibodies with related specificity but of distinct idiotypic composition that may have significance in the use of human monoclonal antibodies in therapy.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.88.24.11120

Authors: A S Kang, T M Jones, D R Burton

Institutions: Scripps Research Institute, University of Sheffield

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Lister Institute of Preventive Medicine, National Center for Research Resources

Award: GENERAL CLINICAL RESEARCH CENTER