Work

Secreted Vago restricts West Nile virus infection in Culex mosquito cells by activating the Jak-STAT pathway

Year: 2012

Type: article

Abstract: Although West Nile virus (WNV) and other arthropod-borne viruses are a major public health problem, the mechanisms of antiviral immunity in mosquitoes are poorly understood. Dicer-2, responsible for the RNAi-mediated response through the C-terminal RNase-III domain, also contains an N-terminal DExD/H-box helicase domain similar to mammalian RIG-I/MDA5 which, in Drosophila, was found to be required for activation of an antiviral gene, Vago. Here we show that the Culex orthologue of Vago (CxVago) is up-regulated in response to WNV infection in a Dicer-2-dependent manner. Further, our data show that CxVago is a secreted peptide that restricts WNV infection by activation of the Jak-STAT pathway. Thus, Vago appears to function as an IFN-like antiviral cytokine in mosquitoes.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.1205231109

Authors: Prasad N. Paradkar, Lee Trinidad, Rhonda Voysey, Jean-Bernard Duchemin, Peter J. Walker

Institutions: Commonwealth Scientific and Industrial Research Organisation, Australian Centre for Disease Preparedness, Animal, Food and Health Sciences

Language: English

Topic: Invertebrate Immune Response Mechanisms

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2003859935

FWCI: 8.086

Cites: 36

Cited by: 348

Related to: 10