Work

Spectral contrast imaging microscopy

Year: 2011

Type: article

Abstract: We introduce a new technique, spectral contrast imaging microscopy (SCIM), for super-resolution microscopic imaging. Based on a novel contrast mechanism that encodes each local spatial frequency with a corresponding optical wavelength, SCIM provides a real-time high-resolution spectral contrast microscopic image with superior contrast. We show that two microscopic objects, separated by a distance smaller than the diffraction limit of the optical system, can be spatially resolved in the SCIM image as different colors. Results with numerical simulation and experiments using a high-resolution United States Air Force target are presented. The ability of SCIM for imaging biological cells is also demonstrated.

Source: Optics Letters

DOI: 10.1364/ol.36.003323

Authors: Sergey A. Alexandrov, Shikhar Uttam, Rajan K. Bista, Yang Liu

Institution: University of Pittsburgh

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Wallace H. Coulter Foundation, National Cancer Institute

Awards: G2631640330, G4296094825