Work

Binding of [ 14 C]Erythromycin to Escherichia coli Ribosomes

Year: 1974

Type: article

Abstract: Erythromycin binding to Escherichia coli ribosomes required K(+) and Mg(2+). Under optimal conditions, the dissociation constant for erythromycin binding to E. coli ribosomes was found to be 1.0 x 10(-8) M and 1.4 x 10(-8) M at 24 C and 5 C, respectively. One molecule of [(14)C]erythromycin was bound to each 70S ribosome at equilibrium. Binding of erythromycin to ribosomes was rapid and reversible. The specific rate constants for the forward and reverse reactions were 1.7 x 10(7) liters per mol per min and 0.15 per min, respectively.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.6.4.474

Author: Sidney Pestka

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2004225393

FWCI: 2.752

Cites: 15

Cited by: 78

Related to: 10