Work

Analysis of a Temperature-Sensitive Mutation Affecting the Integration Protein of Moloney Murine Leukemia Virus

Year: 1993

Type: article

Abstract: We have previously described a series of mutations in the 3' terminus of the pol gene of Moloney murine leukemia virus which adversely affect the establishment of an integrated provirus (Roth, M. J., Schwartzberg, P., Tanese, N., and Goff, S. P., J. Virol. 64, 4709-4717, 1990). While most of the mutations were unconditionally lethal for virus replication, we now demonstrate that one mutation, in6161-12, a 12-base pair linker insertion into the 3' end of the pol gene, causes a temperature-sensitive phenotype. This mutant virus is temperature-sensitive for IN protein function as well as viral replication.

Source: Virology

DOI: 10.1006/viro.1993.1086

Authors: Pamela L. Schwartzberg, Monica J. Roth, Naoko Tanese, Stephen P. Goff

Institutions: Columbia University Irving Medical Center, University of Medicine and Dentistry of New Jersey, Columbia University

Language: English

Topic: T-cell and Retrovirus Studies

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,160

Funder: National Cancer Institute