Vitreoretinal morphology in active ocular toxoplasmosis: a prospective study by optical coherence tomography
Year: 2006
Type: article
Study design: Observational Study
Abstract: OBJECTIVE: To determine whether debris laden phagocytes re-enter the blood circulation from the brain. BACKGROUND: We have postulated that phagocytic leukocytes recruited into the brain to clear away damaged brain tissue re-enter the blood from the brain while containing brain molecules as cargo as a result of their damage clearance function. Interrogation of the cargo in these blood borne cells may represent a novel biomarker strategy that will report pathophysiological activity in the brain. DESIGN/METHODS: studied peripheral blood mononuclear cells (PBMCs) from relapsing -remitting MS patients (RRMS, n=18) and healthy controls (n=12) in order to detect neural antigens carried as cargo. Hypo-osmotic lysates of PBMCs were coated onto ELISA wells at 5ug/ml and probed for the presence of the neuronal microtubule associated protein Tau and the neuronal calcium sequestering protein Hippocalcin 1 like-1 using rabbit polyclonal antibodies and standard assay procedures. RESULTS: Tau was present in 7 of 18 RRMS lysates and 1 of 12 healthy control lysates. Hippocalcin1 like-1 was present in 5 of 18 RRMS lysates and 1 of 12 healthy control lysates. Neither the Tau nor Hippocalcin1 like-1 results were statistically significant by the two tailed Fisher’s exact test. However, positivity for either Tau OR Hippocalcin1 like-1 resulted in 9 of 18 RRMS patients being positive and 1 of 12 healthy controls being positive. Fisher9s exact test: two-tailed p value equals 0.0235. The association between rows (groups) and columns (outcomes) is statistically significant. CONCLUSIONS: The results support the concept of interrogating circulating mononuclear cells for neural antigens as a “window into the brain” that may form the basis of a novel biomarker strategy to develop diagnostic and prognostic aids for Brain injury. Study Supported by: MSDx Inc Disclosure: Dr. Nayak has received personal compensation for activities with MSDx Inc as an employee. Dr. Vollmer has received personal compensation for activities with Cleveland Clinic, Novartis, Mandell Center for Multiple Sclerosis, Teva Neuroscience, Questcor Pharmaceuticals, Biogen Idec, Xenoport, University of Florida PeerView, and Krog & Partners. Dr. Vollmer has received research support from Biogen Idec, Teva Neuroscience, Genzyme Corp., Ono Pharmaceuticals, Elan Pharmaceuticals, Novartis, Avanir Pharmaceuticals, and Janssen Pharmaceutica. Dr. Bomprezzi has received personal compensation for activities with Biogen Idec as a member of a scientific advisory board. Mr. Jacolik has received personal compensation for activities with MSDx Inc.
Source: British Journal of Ophthalmology
Authors: J. L Orefice, R. A Costa, F. Orefice, W. Campos, D. da Costa-Lima, I. U Scott
Language: English
Subfield: Parasitology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2004631909