Work

Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Year: 1975

Type: article

Abstract: Relaxed circular, covalently closed simian virus 40 DNA molecules were associated with the four histones that are present in virions. In electron micrographs the resulting complexes appear twisted, with globular structures (nucleosomes) along the DNA. Incubation with an untwisting extract converts the twisted complexes to relaxed structures. Extraction of the DNA from the relaxed complexes yields supercoiled molecules. The number of superhelical turns in these molecules corresponds to the number of nucleosomes per DNA molecule in the complexes.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.72.5.1843

Authors: J E Germond, B Hirt, P Oudet, M Gross-Bellark, P Chambon

Institution: Swiss Cancer Center Léman

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Centre National de la Recherche Scientifique

OpenAlex ID: W2004837297

FWCI: 17.25

Cites: 31

Cited by: 592

Related to: 10