Work

Yeast Gene for a Tyr-DNA Phosphodiesterase that Repairs Topoisomerase I Complexes

Year: 1999

Type: article

Abstract: Covalent intermediates between topoisomerase I and DNA can become dead-end complexes that lead to cell death. Here, the isolation of the gene for an enzyme that can hydrolyze the bond between this protein and DNA is described. Enzyme-defective mutants of yeast are hypersensitive to treatments that increase the amount of covalent complexes, indicative of enzyme involvement in repair. The gene is conserved in eukaryotes and identifies a family of enzymes that has not been previously recognized. The presence of this gene in humans may have implications for the effectiveness of topoisomerase I poisons, such as the camptothecins, in chemotherapy.

Source: Science

DOI: 10.1126/science.286.5439.552

Authors: Jeffrey J. Pouliot, Kevin C. Yao, Carol A. Robertson, Howard A. Nash

Institutions: National Institutes of Health, National Institute of Mental Health

Language: English

Topic: Cancer therapeutics and mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2005110566

FWCI: 5.373

Cites: 16

Cited by: 384

Related to: 10