Work

Sequential Gene Silencing Using Wavelength‐Selective Caged Morpholino Oligonucleotides

Year: 2014

Type: article

Abstract: Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-selective illumination have been used to sequentially inactivate organismal gene function. The efficacy of these reverse-genetic chemical probes has been demonstrated in zebrafish embryos, and these reagents have been employed to examine the mechanisms of mesoderm patterning.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201405355

Authors: Sayumi Yamazoe, Qingyang Liu, Lindsey E. McQuade, Alexander Deiters, James K. Chen

Institutions: Stanford Medicine, Stanford University, University of Pittsburgh

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Center for Chronic Disease Prevention and Health Promotion, Eunice Kennedy Shriver National Institute of Child Health and Human Development