Year: 2005
Type: article
Abstract: C150 crystals, mounting inside a hutch and data acquisition.Obtaining X-ray quality crystals might require setting up thousands of crystallization trials on different conditions for protein solutions and precipitants.To this end, we have developed a largescale protein crystallization system with the capability of setting up 240,000 crystallization trials per day.This system allows efficient crystallization trials of large protein complexes and structure-based drug design.It includes a crystallization tray supplier, a dispensing system using disposable tips which are specially designed for high dispensing accuracy, a sealing system, an observation system for taking images of drops in wells according to a pre-programmed schedule and storing them to the file server, incubators and a carrying robot which moves the trays between these components.Users can access the file server from anywhere in the world through a Web browser for checking the status of their crystal growth.We will also describe a crystal harvesting system and automated sample exchange robots based on the SAM (SSRL Automated Mounting) system as part of our efforts in automating X-ray structure determination.
Authors: E. E. Abola, P. Kuhn, R. Stevens
Institution: Scripps Research Institute
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2005930969