Work

Synthesis and biological characterization of monocyte‐derived neutrophil chemotactic factor

Year: 1988

Type: article

Abstract: MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

Source: FEBS Letters

DOI: 10.1016/0014-5793(88)80078-4

Authors: Shuji Tanaka, Elizabeth A. Robinson, Teizo Yoshimura, Kouji Matsushima, Edward J. Leonard, Ettore Appella

Institutions: National Institutes of Health, National Cancer Institute, Frederick National Laboratory for Cancer Research

Language: English

Topic: Chemical Synthesis and Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Foundation for Promotion of Cancer Research