Year: 1983
Type: article
Abstract: The interaction of coat proteins from coated vesicles with model lipid membranes was examined using small unilamellar vesicles of dipalmitoylglycerophosphocholine as model membranes. Changes in membrane permeability were measured by the leakage of entrapped fluorescent dye, carboxyfluorescein. Both clathrin and the 55000-Da protein were found to be active. Density gradient centrifugation showed the formation of an irreversible protein-lipid complex. Dynamic light-scattering measurements showed that this complex is significantly larger than the original vesicles, suggesting that fusion is induced. The effects of pH, urea, Tris and ionic strength were studied and the possible biological relevance of the results is discussed.
Source: European Journal of Biochemistry
Authors: Evelyn RALSTON, Jane ROBINSON, Robert FINSY, Yves ENGELBORGHS
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2006356982