Work

Folding DNA Origami from a Double-Stranded Source of Scaffold

Year: 2009

Type: article

Abstract: Combined heat and chemical denaturation of double-stranded DNA scaffold strands in the presence of staple strands, followed by a sudden temperature drop and then stepwise dialysis to remove the chemical denaturant, leads to self-assembly of two distinct DNA-origami structures.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja902569x

Authors: Björn Högberg, Tim Liedl, William M. Shih

Institutions: Harvard University, Dana-Farber Cancer Institute

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Deutscher Akademischer Austauschdienst, Vetenskapsrådet, National Institutes of Health

Award: NMR Structure Determination of Membrane Proteins Enabled by DNA Nanotubes